Characterization of γδT cells in lung of Plasmodium yoelii-infected C57BL/6 mice

Abstract Background Malaria has high morbidity and mortality rates in some parts of tropical subtropical countries. Besides respiratory metabolic function, lung plays a role immune system. γδT cells have multiple functions producing cytokines chemokines, regulating the response by interacting with other cells. It remains unclear about mice infected malaria parasites. Methods Flow cytometry (FCM) was used to evaluate frequency effects on phenotype function B T Plasmodium yoelii -infected wild-type (WT) or γδTCR knockout (γδT KO) mice. Haematoxylin-eosin (HE) staining observe pathological changes lungs. Results The percentage absolute number increased after infection ( p < 0.01). More were expressing CD80, CD11b, PD-1 post-infection 0.05), while less CD34, CD62L, CD127 0.05). percentages IL-4 + , IL-5 IL-6 IL-21 IL-1α IL-17 but IFN-γ-expressing decreased 0.05) post-infection. lungs KO not obvious compared WT proportion CD3 numbers cells, CD4 CD8 exhibited no significant difference surface molecular expression > While, There total, CD69 ICOS CD80 between Conclusions content, phenotype, C57BL/6 changed infection. contribute cell progress